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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
 
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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Article DOI: 10.3791/1167-v • 15:43 min • March 6th, 2009
March 6th, 2009 •
1, 2, 3, 2
1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute

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This procedure demonstrates the purification and in vitro expansion of antigen specific CD4+ T cells from whole peripheral blood and their visualization using MHC class II tetramers. Tetramers permit the direct visualization of T cells with a single antigen specificity and defined MHC class II restriction.

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CD4+ T Cells MHC Class II Tetramers Antigen Specific Flow Cytometry Peptide Loaded MHC T-cell Receptor Streptavidin Staining Reagents In Vitro Amplification Mononuclear Cells Peripheral Blood Ficoll Underlay Negative Selection Biotinylated Antibody Cocktail Magnetic Beads Adherent Cells Antigen Presenting Cells IL-2 Surface Antibodies Flow Cytometry Analysis
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