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DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation
 
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DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation

Article DOI: 10.3791/1352-v • 15:15 min • September 18th, 2009
September 18th, 2009 •
1, 1, 1, 1, 1
1Department of Microbiology and Immunology, University of British Columbia - UBC

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We describe a method for extraction of high molecular weight genomic DNA from planktonic biomass concentrated on 0.22 μm Sterivex filters, followed by cesium chloride density gradient centrifugation for purification.

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DNA Extraction High Molecular Weight Genomic DNA Planktonic Biomass Sterivex Filters Storage/lysis Buffer -80°C Storage Cesium Chloride Density Gradient Centrifugation Incubation Steps Liberate DNA Remove RNA Phenol:Chloroform Extraction Chloroform Extraction Centrifugation Protein Removal Cell Membrane Removal Aqueous DNA Extract Buffer Exchange Purification Cesium Chloride Density Gradient Sample Limitation Protocol Time Centrifugation Equipment Hybridization Ovens
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