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一个<em在体外皮肤刺激测试（SIT），使用的表皮重建人表皮（RHE）型号
JoVE Journal
Biologia
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JoVE Journal Biologia
An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
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一个<em在体外皮肤刺激测试（SIT），使用的表皮重建人表皮（RHE）型号

DOI:
10.3791/1366-v

•

21:16 min

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July 13, 2009

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, , , ,
1MatTek Corporation

Capítulos

  • 00:05Título
  • 01:32Introduction
  • 02:07The EpiDerm – Reconstructed Human Epidermal Model
  • 04:02Day 0 – Preparing for the Assay
  • 05:21Day 0 – Tissue Conditioning
  • 08:09Day 1 – Chemical Exposure
  • 15:28Day 2 – Media Change
  • 16:03Day 3 – MTT Viability Assay
  • 19:55Representative Results
  • 20:33Conclusion

Summary

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Tadução automática

在这段视频中，我们展示了开发和验证的表皮皮肤刺激性试验（表皮薛）<em>在体外</em>化学品的皮肤刺激测试，包括化妆品和​​药品的成分。

Tags

In Vitro Skin Irritation TestEpiDerm Reconstructed Human Epidermal ModelEpiDerm SITIn Vitro Skin Irritation TestingCosmetic IngredientsPharmaceutical IngredientsGHS Category 2MTT Viability AssayTest ChemicalsPositive ControlNegative Control60-minute Exposure42-hour Post-incubationIncubatorCytokines

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