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Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
 

Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

Article DOI: 10.3791/1512-v • 10:43 min • September 4th, 2009
September 4th, 2009 •
1, 1, 1, 1
1Institute of Biomedical Sciences, Federal University of Rio De Janeiro-UFRJ

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Karyotyping is a simple and useful technique widely used for detecting genetic alterations. Here we describe a step by step protocol for chromosome spread preparation of human embryonic stem cells for monitoring the chromosomal status of these cells maintained in culture.

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Chromosomal Spread Preparation Human Embryonic Stem Cells Karyotyping Stable Diploid Karyotype Chromosomal Anomalies Culture Conditions Enzymatic Dissociation Chemical Dissociation Manual Dissection Feeder Cells Genetic Integrity Cellular Physiology Embryogenesis Studies Drug Testing Therapeutic Purposes Chromosomal Changes Carcinogenesis Chromosome Spreads G-banding FISH SKY Techniques CGH Techniques Translocations Aneuploidies
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