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A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
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Biologia
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JoVE Journal Biologia
A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

DOI:
10.3791/1649-v

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07:55 min

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January 26, 2010

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, , , , ,
1Department of Surgery,University of Illinois, Chicago, 2Department of Bioengineering,University of Illinois, Chicago

Capítulos

  • 00:00Título
  • 00:34Introduction
  • 01:10Microfabrication of 3-layer Microfluidic Perifusion Device
  • 02:18Simultaneous Perifusion and Imaging of Mouse Islets
  • 04:45Islet Perifusion
  • 06:55Simultaneous Monitoring of Multiple Parameters in Stimulated Islets
  • 07:23Conclusion

Summary

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A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes.

Tags

Microfluidic Islet Perifusion DeviceDynamic Insulin SecretionFluorescence ImagingCalcium InfluxMitochondrial Potential ChangesMicroscale WellsPerifusion ChamberInlet-mixing ChannelGlucose GradientsLinear ShapeBell ShapeSquare Shape

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