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Measuring the Induced Membrane Voltage with Di-8-ANEPPS
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Biologia
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JoVE Journal Biologia
Measuring the Induced Membrane Voltage with Di-8-ANEPPS

Measuring the Induced Membrane Voltage with Di-8-ANEPPS

DOI:
10.3791/1659-v

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05:52 min

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November 19, 2009

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, ,
1Department of Biomedical Engineering, Faculty of Electrical Engineering,University of Ljubljana

Capítulos

  • 00:06Introduction
  • 01:49Preliminary Steps
  • 02:28Loading the cells with di-8-ANEPPS
  • 03:21Experiment and image acquisition
  • 04:35Image processing and analysis

Summary

Tadução automática

Tadução automática

External electric field induces a voltage on the membrane of a cell, termed the induced membrane voltage (ΔΦ). By using the potentiometric dye di-8-ANEPPS, it is possible to measure the ΔΦ noninvasively. This video shows the protocol for measuring ΔΦ using di-8-ANEPPS.

Tags

Induced Membrane VoltageDi-8-ANEPPSCellElectric FieldCharge RedistributionCell MembraneVoltageInduced Transmembrane VoltageInduced Transmembrane Potential DifferenceDelta PhiResting VoltagePotentiometric Fluorescent DyesVariationsNoninvasivelyLipid BilayerFluorescence IntensityProtocolCell ShapeAmplitudeSpatial Distribution

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