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收获的小鼠精子和人工授精

JoVE Journal
Biologia

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JoVE Journal Biologia

Harvesting Sperm and Artificial Insemination of Mice

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收获的小鼠精子和人工授精

DOI:

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10:00 min

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April 28, 2007

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Amanda R. Duselis, Paul B. Vrana

¹Dept. of Biological Chemistry,University of California, Irvine (UCI)

Capítulos

00:06Introduction
01:09Tools and Reagents
02:17Dissection procedure
04:43Removal of epididymis
07:58Visualization of sperm
08:20Insemination of females
09:27Conclusion

Summary

Tadução automática

此协议表明，从男性的睾丸中提取精子，然后inseminating雌性小鼠的方法。此过程需要精确的时间是在发育的研究，以及转基因工作时非常有用。

Tags

Sperm Harvesting Artificial Insemination Mice Peromyscus Species Pesquisa Model Protocol Testes Extraction Sperm Isolation Milk Mixture Female Reproductive Tract Fertilization Embryo Development Embryo Timing Rodent Species Mating Assessment Transgenic Work Reduced Animal Usage Peromyscus Sy

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