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Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
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Biologia
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JoVE Journal Biologia
Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

DOI:
10.3791/1915-v

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14:58 min

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June 02, 2010

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, ,
1Department of Biomedical Engineering,Rutgers University

Capítulos

  • 00:00Título
  • 00:50Introduction
  • 01:26Protocol
  • 02:18Preparing the Optical Setup
  • 05:26Loading the Filterbank and Using the Setup to Acquire Filtered-Background Images
  • 06:57Plating the Cells
  • 09:44Performing Optical Scatter Microscopy
  • 11:53Representative Images
  • 14:28Conclusion

Summary

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We demonstrate a dark-field microscopy method based on Gabor-like filtering to measure subcellular dynamics within single living cells. The technique is sensitive to alterations in the structure of organelles, such as mitochondrial fragmentation.

Tags

Optical Scatter MicroscopyTwo-dimensional Gabor FiltersSubcellular TextureOrganelle MorphologyLabel-free Optical MicroscopyNanoscale ChangesOrganelle Size And ShapeStructure-function RelationshipOrganelle DynamicsBiological ProcessesProgrammed Cell DeathCellular DifferentiationMicroscopy PlatformsDisseminated To Individual LaboratoriesUnrestricted AccessSubcellular Structure CharacterizationOptical Gabor FiltersNanoscale SensitivityMorphological AttributesNon-spherical Subcellular OrganellesElastic Scattering ContrastInverse Scattering ModelMie TheoryMorphometric Measurements

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