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In situ Quantification of Pancreatic Beta-cell Mass in Mice
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Biologia
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JoVE Journal Biologia
In situ Quantification of Pancreatic Beta-cell Mass in Mice

In situ Quantification of Pancreatic Beta-cell Mass in Mice

DOI:
10.3791/1970-v

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09:50 min

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June 07, 2010

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, ,
1Department of Medicine,University of Chicago

Capítulos

  • 00:00Título
  • 00:57Introduction
  • 01:23Preparation of Specimens
  • 04:19Imaging and Quantifying the Beta-Cell Area in the Intact Pancreas
  • 07:58Slice Image Analysis of Beta Cells in an Intact Adult Pancreas
  • 09:27Conclusion

Summary

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Tadução automática

The following protocol outlines the process of pancreatic dissection for virtual slice imaging, and the subsequent quantification of all GFP-tagged beta-cells in the entire pancreas.

Tags

In Situ QuantificationPancreatic Beta-cell MassMiceSpecific Cell PopulationsHealth And DiseaseBiomedical ResearchMonitoringBeta-cell ProliferationIslet GrowthMethod DevelopmentFluorescence-tagged Beta-cellsImageJ MacroPancreatic DissectionVirtual SliceGFP-tagged Beta-cells ExaminationQuantificationTotal Beta-cell AreaIslet NumberSize DistributionSpecific Parameters And LocationsSmall Clusters Of Beta-cellsDistribution PlottingThree DimensionsInformation From Distribution AnalysisQuantitative ComparisonQualitative Comparison

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