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JoVE Journal Developmental Biology

使用FACS分选的小鼠卫星细胞的核酸酶分析在靶标下进行切割和释放

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使用FACS分选的小鼠卫星细胞的核酸酶分析在靶标下进行切割和释放

Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis

Article DOI: 10.3791/200376-v • 04:25 min • July 7th, 2023

July 7th, 2023 •

Kamar Ghaibour*¹, Joe Rizk*¹, Claudine Ebel¹, Tao Ye¹, Muriel Philipps¹, Valérie Schreiber¹, Daniel Metzger¹, Delphine Duteil¹

¹CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258, Université de Strasbourg

* These authors contributed equally

Tags

CUT&RUN分析， FACS分离的小鼠卫星细胞，干细胞场，小细胞群，高效实验方案，荧光激活细胞分选，肢体肌肉，结构蛋白，碎浆，培养基，分散酶， I型胶原酶，细胞过滤器， FACS缓冲液，活力染色剂，免疫染色卫星细胞， Triton X-100，刀豆球蛋白A磁珠，转录因子，组蛋白修饰，蛋白A-微球菌核酸酶，染色质切割， CaCl2， DNA提取，图书馆，生物信息学分析

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