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Oral Submucous Fibrosis Tissue Sample Preparation for Hematoxylin-Eosin Staining (Video) | JoVE

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Oral Submucous Fibrosis Tissue Sample Preparation for Hematoxylin-Eosin Staining (Video) | JoVE

Capturing Oral Submucous Fibrosis Tissue Samples for Spatial-Omics Analysis

Article DOI: 10.3791/200508-v • 02:14 min • August 11th, 2023

August 11th, 2023 •

Xinjia Cai^1,2, Heyu Zhang³, Jianyun Zhang^1,2, Tiejun Li^1,2

¹Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, ²Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), ³Central Laboratory, Peking University School and Hospital of Stomatology

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Isolierung Zellen morphologische Informationen räumliche Informationen orale submuköse Fibroseproben Laser-Capture-Mikrodissektion potentiell maligne Erkrankung Atrophie Epithel Fibrose Lamina propria Submukosa histopathologische Objektträger Epitheldysplasie seneszente Fibroblasten maligne Transformation molekulare Mechanismen Heterogenität orales Plattenepithelkarzinom spezifische molekulare Mechanismen Formalinfixierte Paraffin-eingebettete Gewebeobjektträger Mikroskop mikroskalige Dysplastik Gewebe atrophisches Epithelgewebe fibrotisches subepitheliales Gewebe Genomsequenzierung Transkriptomsequenzierung Genomdaten Transkriptomdaten

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