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从人胰腺肿瘤细胞中分离细胞核进行多组测序
 
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从人胰腺肿瘤细胞中分离细胞核进行多组测序

Isolation of Nuclei for Multiome Sequencing in Solid Tumor Specimen

Article DOI: 10.3791/200649-v • 02:13 min • October 13th, 2023
October 13th, 2023 •
1, 1, 1, 1, 1, 1
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University

Tags

优化的细胞核分离、新鲜肿瘤标本、冷冻肿瘤标本、多组学测序、单细胞 RNA 测序、ATAC 测序、肿瘤细胞异质性、转化癌症研究、测序数据质量、消化条件优化、细胞产量最大化、实体瘤结缔增生性基质、细胞释放、脆性细胞、异质细胞类型、总细胞群支持、核分离条件优化、裂解时间、试剂类型/比率、10x 基因组学多组测序平台
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