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生产转基因非洲爪蟾限制性内切酶介导的整合和核移植
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Biologia
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JoVE Journal Biologia
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
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生产转基因非洲爪蟾限制性内切酶介导的整合和核移植

DOI:
10.3791/2010-v

•

09:48 min

•

August 21, 2010

•

,
1The Healing Foundation Centre, Faculty of Life Sciences,University of Manchester, 2Department of Developmental Biology,Washington University School of Medicine

Capítulos

  • 00:04Título
  • 00:11Introduction
  • 01:21Sperm Nuclei Preparation
  • 03:33Determinining Nuclei Yield
  • 04:15Preparation of High Speed Extract
  • 06:58Transgenesis Reaction and Nuclear Transfer
  • 09:05Representative Transgenic Embryo
  • 09:24Conclusion

Summary

Tadução automática

Tadução automática

这个视频协议演示了用于产生转基因的方法<em>非洲爪蟾</em>由引进到精子细胞核转基因细胞核移植到未受精卵。

Tags

Transgenic Xenopus LaevisRestriction EnzymeNuclear TransplantationGene ExpressionTransgenesis ApproachSperm NucleiEgg ExtractLinearized Plasmid DNAChromosomal BreaksRecombinationEmbryonic CleavageChimeric Embryos

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