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CENP-E 敲除 HeLa 细胞的转染、分离和筛选

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CENP-E 敲除 HeLa 细胞的转染、分离和筛选

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

Article DOI: 10.3791/201002-v • 04:10 min • June 23rd, 2023

June 23rd, 2023 •

Meng-Fei Xu^1,2, Jie Chen^1,2, Yue Xu^1,2, Jing-Lian Zhang^1,2, Yi Zhou^1,2, Jie-Jie He^1,2, Shan Wu^1,2, Ya-Lan Wei^3,4, Zhen-Yu She^1,2

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, ⁴Medical Research Center, Fujian Maternity and Child Health Hospital

Tags

生物学，第 196 期，CENP-E 敲除、着丝粒相关蛋白-E、驱动蛋白、动粒微管捕获、染色体比对、纺锤体组装检查点、HeLa 细胞、基于表型的筛选、染色体错位、BUB1 有丝分裂检查点丝氨酸/苏氨酸激酶 B （BubR1）、有丝分裂缺陷

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