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Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
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Neurociência
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JoVE Journal Neurociência
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

DOI:
10.3791/2086-v

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06:32 min

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September 01, 2010

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, , ,
1Department of Cell and Developmental Biology,Vanderbilt University

Capítulos

  • 00:00Título
  • 00:59Introduction
  • 01:20Micro-dissection of Tumor-bearing Cerebellum and Dissociation of Tumor Tissue
  • 03:34Enrichment, Maintenance, and Expansion of Medulloblastoma Tumor Cells by Serial Passages
  • 04:26Immunofluorescent Staining of Cultured Medulloblastoma Cells
  • 04:53Morphology, Lineage Differentiation, and Expression of Stem Cells Markers
  • 06:17Conclusion

Summary

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This protocol describes the isolation, enrichment, and maintenance of medulloblastoma tumor stem cells derived from mutant mice with ectopic Sonic hedgehog pathway activity.

Tags

IsolationEnrichmentMaintenanceMedulloblastoma Stem CellsBrain TumorsNeurosphere AssaysNeural Stem CellsTumorigenesisTumor ProgressionShh SignalingCerebellar DevelopmentCancer Stem Cells

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