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Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation
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Neurociência
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JoVE Journal Neurociência
Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation

Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation

DOI:
10.3791/2107-v

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09:38 min

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September 12, 2010

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,
1Neurosciences Graduate Program,University of Southern California, 2Zilkha Neurogenetic Institute, Department of Physiology and Biophysics,University of Southern California Keck School of Medicine

Capítulos

  • 00:00Título
  • 00:55Introduction
  • 01:08Preparing Electrodes and Solutions
  • 02:25Setup of the Recording Apparatus
  • 04:29Eye Dissection and Slicing
  • 07:42Recording
  • 08:44Representative Results: Light-evoked from Retinal Neurons
  • 09:08Conclusion

Summary

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Here we describe a procedure for generating dark-adapted slices of the mouse retina for electrophysiological recordings.

Tags

Patch Clamp RecordingsMouse Retinal NeuronsDark-adapted Slice PreparationVisual PigmentRetinal PhotoreceptorsIntracellular EventsCyclic-nucleotide-gated ChannelsMembrane PotentialNeurotransmitter ReleaseElectrophysiological RecordingsRetinal Slice PreparationsRazor BladeFilter PaperLow Gelling Temperature AgarVibrating MicrotomeSurface DamageLight-evoked ResponsesInfrared LightBleaching Visual Pigment

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