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Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
 
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Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Article DOI: 10.3791/2134-v • 10:44 min • December 3rd, 2010
December 3rd, 2010 •
1, 1
1Department of Biological Sciences, University of Illinois Chicago - UIC

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In this protocol we demonstrate the expression, solubilization, and purification of a recombinantly expressed membrane protein, MexB, as a soluble protein detergent complex. MexB is a multidrug resistance membrane transporter from the opportunistic bacterial pathogen Pseudomonas aeruginosa.

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Membrane Transporter MexB Multidrug Resistance Protein Multidrug Resistance Drug Efflux Pumps Pseudomonas Aeruginosa Tripartite Complexes Inner Membrane Proton-drug Antiporter Outer Membrane Channel Periplasmic Linker Protein Transmembrane Proteins Protein Detergent Complex (PDC) Detergent Solubilization Purification
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