Journal
/
Imunologia e Infecção
/
在调节Toll样受体激活Neu1唾液酸酶活动的检测
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
Please note that all translations are automatically generated. Click here for the English version.

在调节Toll样受体激活Neu1唾液酸酶活动的检测

DOI:
10.3791/2142-v

•

09:04 min

•

September 07, 2010

•

, , , , , ,
1Department of Microbiology and Immunology,Queen’s University – Kingston, Ontario

Capítulos

  • 00:00Título
  • 01:06Introduction
  • 01:48Resurrecting Frozen Macrophage Cells
  • 02:50Plating Cells for the Sialidase Assay
  • 04:42Assaying for Sialidase Activity
  • 06:50Sialidase Assay Results
  • 08:36Conclusion

Summary

Tadução automática

Tadução automática

唾液酸酶检测是一个简单的技术方法，将澄清新颖的TLR传感器，微生物感染和参与活巨噬细胞的细胞表面受体水平的炎症性疾病的分子机制（S）。

Tags

Neu1 Sialidase ActivityTOLL-like Receptor ActivationMammalian Toll-like ReceptorsPathogen-associated Molecular PatternsInfectious DiseasesInflammatory DiseasesAutoimmune DiseasesStructural Integrity Of Sensor ReceptorsLigand InteractionsSignaling ComponentsImmunological ProtectionDisease ModificationLigand-induced TLR ActivationNeu1 Sialidase ActivationNeurotrophin ReceptorsTrkATrkBMatrix Metalloproteinase-9 (MMP-9)Sialidase Assay

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

胶粘剂基于磁带的采样和荧光组合原位杂交快速检测沙门氏菌新鲜农产品

定量成像小样本人体血液中单核细胞和树突状细胞系特定的Toll样受体介导的信号

唾液，唾液腺，淋巴收集肩突硬蜱蜱

定量检测抗体诱导补体激活的方法对红血细胞

激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量

小鼠腹腔巨噬细胞的分离，以贯彻基因表达分析后Toll样受体刺激

分离及小鼠淋巴细胞的激活

人全血中NK细胞杀伤活性的流式细胞仪分析

小鼠骨髓源性巨噬细胞 Inflammasome 活化和 Pyroptotic 细胞死亡的检测

沙门氏菌筛选的高通量平台.

Read Article