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共培养模型绿脓杆菌现场人类呼吸道细胞生长的生物膜
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
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共培养模型绿脓杆菌现场人类呼吸道细胞生长的生物膜

DOI:
10.3791/2186-v

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11:21 min

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October 06, 2010

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, , ,
1Department of Physiology,Dartmouth College, 2Department of Biology,Indiana University Purdue University Indianapolis

Capítulos

  • 00:00Título
  • 00:54Introduction
  • 01:18Static Co-culture Biofilm Model
  • 03:41Flow Cell Co-culture Biofilm Model
  • 08:36Representative Results
  • 10:52Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了成长的不同方法<em>绿脓杆菌</em>对培养的人类呼吸道上皮细胞的生物膜。这些协议可以适应研究生物膜形成的不同方面，包括可视化的生物膜，生物膜染色，测量菌落形成单位（CFU）的生物膜，研究生物膜的细胞毒性。

Tags

Co-culture ModelsPseudomonas Aeruginosa BiofilmsHuman Airway CellsCFBE CellsStatic Co-culture Biofilm ModelFlow Cell Co-culture Biofilm ModelArginineBacterial BiofilmsEpithelial CellsBiofilm DevelopmentGlass CoverslipPeristaltic PumpFresh MediumVisualizationGreen Fluorescent Protein (GFP)Cystic Fibrosis (CF) Lung

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