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Immunology and Infection

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JoVE Journal Immunology and Infection
Enzyme-linked Immunospot Assay (ELISPOT)
 
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Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Article DOI: 10.3791/2221-v • 06:14 min • November 23rd, 2010
November 23rd, 2010 •
*1, *1, 2, 1,2
1Department of Medicine, Vanderbilt University School of Medicine, 2Department of Microbiology and Immunology, Vanderbilt University School of Medicine
* These authors contributed equally

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Identification of microbial targets of adaptive immunity in idiopathic diseases can be accomplished by the use of the enzyme-linked immunospot assay.

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Enzyme-linked Immunospot Assay ELISPOT Cellular Immune Responses Microbial Antigens Th-1 Immune Response Diagnostic Tool Immunosuppressed Populations HIV Infection Transplantation Steroid Use Th1 Or Th2 Immune Response Peripheral Blood Bronchoalveolar Lavage Cerebral Spinal Fluid Ascites Epitopes Within Microbial Proteins Immune Responses Over Time Dual Color ELISPOT Assays Cytokines Extrapulmonary Tuberculosis Infectious Antigens Autoimmunity
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