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Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
JoVE Journal
Biologia
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JoVE Journal Biologia
Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

DOI:
10.3791/2429-v

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05:39 min

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November 07, 2010

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, , ,
1Department of Molecular Physiology and Biophysics,Baylor College of Medicine

Capítulos

  • 00:00Título
  • 01:15Cell Staining
  • 04:05Data Aquisition
  • 04:39Upregulation of Surface GLUT-4 Receptor in Response to Insulin on Myoblasts from Healthy or Insulin-resistant Individuals
  • 05:21Conclusion

Summary

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This protocol describes a rapid technique to quantify the translocation of GLUT4 from the cytoplasm to the plasma membrane of cells by flow cytometry.

Tags

Quantitative MeasurementGLUT4 TranslocationPlasma MembraneFlow CytometryGlucoseEnergy SourceBlood ConcentrationInsulin ReleaseType-2 DiabetesObesityInsulin ResistanceGlucose HomeostasisMechanismsTreatment StrategiesGLUT FamilyGlucose TransportersGLUT4 TransporterSkeletal MuscleInsulin ReceptorVesiclesCytoplasmImmunocytochemistryFluorophore-conjugated AntibodiesTechniqueQuantifyTotal Amounts Of GLUT4 Translocation

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