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Flow Cytometry Purification of Mouse Meiotic Cells
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Biologia
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JoVE Journal Biologia
Flow Cytometry Purification of Mouse Meiotic Cells

Flow Cytometry Purification of Mouse Meiotic Cells

DOI:
10.3791/2602-v

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10:43 min

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April 15, 2011

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, ,
1Genome Plasticity Laboratory, Department of Cancer Biology,The Scripps Research Institute, 2Flow Cytometry Core,The Scripps Research Institute

Capítulos

  • 00:05Título
  • 02:03Testis Dissociation and Hoechst 33342 Staining of Testicular Cells
  • 06:03FACS Setup and Purification
  • 08:30Results of Flow Cytometry Purification
  • 09:58Conclusion

Summary

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An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.

Tags

Flow CytometryPurificationMouse Meiotic CellsTestisDifferentiation ProgramsElutriationStaputBSA GradientPercoll GradientCell SizeCell DensityMeiotic FractionsMolecular AnalysesFACSHoechst 33342 StainingGerm CellsRound SpermatidsViable CellsMeiosis ProgressionGene ExpressionNucleosome Occupancy

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