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流动小鼠减数分裂细胞的流式细胞仪净化
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Biologia
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JoVE Journal Biologia
Flow Cytometry Purification of Mouse Meiotic Cells
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流动小鼠减数分裂细胞的流式细胞仪净化

DOI:
10.3791/2602-v

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10:43 min

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April 15, 2011

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, ,
1Genome Plasticity Laboratory, Department of Cancer Biology,The Scripps Research Institute, 2Flow Cytometry Core,The Scripps Research Institute

Capítulos

  • 00:05Título
  • 02:03Testis Dissociation and Hoechst 33342 Staining of Testicular Cells
  • 06:03FACS Setup and Purification
  • 08:30Results of Flow Cytometry Purification
  • 09:58Conclusion

Summary

Tadução automática

Tadução automática

一种有效的方法，从小鼠睾丸中获得高度纯化的可行减数分裂分数描述，一个精细胞分解协议相结合的荧光激活细胞分选（FACS）。这种方法需要利用DNA含量和核密度的离散减数分裂分数的差异。

Tags

Flow CytometryPurificationMouse Meiotic CellsTestisDifferentiation ProgramsElutriationStaputBSA GradientPercoll GradientCell SizeCell DensityMeiotic FractionsMolecular AnalysesFACSHoechst 33342 StainingGerm CellsRound SpermatidsViable CellsMeiosis ProgressionGene ExpressionNucleosome Occupancy

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