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A β-glucuronidase (GUS) Based Cell Death Assay
 
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A β-glucuronidase (GUS) Based Cell Death Assay

Article DOI: 10.3791/2680-v • 07:36 min • May 6th, 2011
May 6th, 2011 •
1, 1, 1
1Department of Plant Pathology and Microbiology, Institute for Plant Genomics and Biotechnology, Texas A&M University

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Programmed cell death assays commonly used in mammalian systems such as DNA laddering or TUNEL assays, are often difficult to reproduce in plants. In combination with a GUS reporter system, we propose a rapid, plant based transient assay to analyze the potential death properties of specific genes.

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A β-glucuronidase (GUS) Transient Plant Expression System Reporter Gene Positive Regulators Of Cell Death N. Benthamiana Leaves Co-infiltration 35S Driven Expression Cassette Gene Analysis GUS Vector PCAMBIA 2301 Agrobacterium Strain LBA4404 Live Cells Marker Gene Anti-apoptotic Genes Pro- And Anti-death Players Bcl-2 Associated AthanoGene (BAG) Family Mammalian Inducers Of Cell Death BAX Post-translational Modification/activation Of Proteins Rapid And Sensitive Plant Based Method
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