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Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
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Chapters
- 00:05Title
- 01:24Prep Work: Dished, Chambers, Blades and Solutions
- 02:47Isolation of Retinas
- 04:49Isolation of Single Photoreceptor Cells
- 06:17Fluorescence Imaging
- 09:29Morphology and Health of Isolated Photoreceptor Cells
- 10:53Conclusion
A method is described for the preparation of single living photoreceptor cells from different vertebrate species for fluorescence imaging. The method can be used to image the fluorescence of endogenous fluorophores, such as NADH or vitamin A, or that of exogenously added fluorescent dyes sensitive to Ca2+ or other factors.
Tags
Living Isolated Vertebrate Photoreceptor CellsFluorescence ImagingPhototransductionRod PhotoreceptorsCone PhotoreceptorsOuter SegmentVisual PigmentChromophoreOpsinPhoton AbsorptionPhotoisomerizationConformational ChangeCascade Of ReactionsMembrane PotentialElectrical SignalRecovery ProcessDeactivation Of IntermediatesCa2+ ModulationAdaptation To Background LightRegeneration Of Visual Pigment
