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1Department of Genetics,UMR_S 968, Institut de la Vision, 2Department of Visual Information,UMR_S 968, Institut de la Vision, 3Exploratory Team,UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06,UMR_S 968, Institut de la Vision, 5INSERM, U968,Institut de la Vision, 6CNRS, UMR_7210,Institut de la Vision
Chapters
- 00:05Title
- 01:40Preparations for Retina Sectioning
- 02:46Isolating and Flattening the Retina
- 04:17Preparing the Retina for Sectioning and Sectioning
- 06:35Dissociating and Culturing Photoreceptor Cells
- 09:03Characterizing the Cultured Photoreceptors
- 10:21Conclusion
Neural retina of a mouse aged 8 days is on top of a 4% gelatin block. After isolation of the photoreceptor layer (200 µm) by vibratome, the photoreceptors are seeded after mechanical and enzymatic dissociation for culture. The photoreceptor layer can be used for molecular, biochemical analyses or transplantation.
