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Vibratome Sectioning for Enhanced Preservation of the Cytoarchitecture of the Mammalian Organ of Corti
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Chapters
- 00:05Tile
- 01:22Dissection and Fixation of Inner Ears
- 04:07Mounting the Sections for Confocal Microscopy
- 04:53Antibody Staining and Confocal Microscopy of Pillar Cells in the Organ of Corti
- 05:28Conclusion
A simple procedure of vibratome sectioning the organ of Corti, followed by immunohistochemistry and confocal microscopy is described. This procedure allows for improved preservation of the fine cytoarchitecture of the mammalian organ of Corti, and consequently allows for accurate quantification of cell types.
Tags
Vibratome SectioningCytoarchitectureMammalian Organ Of CortiCellular MosaicMechanosensory Hair CellsNonsensory Supporting CellsCross-sectioningHistological ProceduresParaffin SectioningCryo-sectioningImmunohistochemical StainingVibratome SectionsConfocal MicroscopyImmunohistochemical AnalysisMutant Organs Of Corti
