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In vitro Reconstitution of the Active T. castaneum Telomerase
 

In vitro Reconstitution of the Active T. castaneum Telomerase

Article DOI: 10.3791/2799-v • 09:26 min • July 14th, 2011
July 14th, 2011 •
1, 1, 1
1Gene Expression and Regulation, The Wistar Institute, University of Pennsylvania

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Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more than a decade. Here, we present methods for the isolation of the recombinant Tribolium castaneum TERT (TcTERT) and the reconstitution of the active T. castaneum telomerase ribonucleoprotein (RNP) complex in vitro.

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Telomerase T. Castaneum Catalytic Subunit Isolation Recombinant Telomerase Ribonucleoprotein In Vitro Reconstitution DNA Repeats Telomeres Reverse Transcriptase Linear Chromosomes End-to-end Fusion Degradation DNA Replication Hayflick Limit Somatic Cells Cancer Cells Cell Immortality Protein Subunit Integral RNA Component Template Synthesis Crystal Structure
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