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慢病毒介导的击倒在体外红细胞

JoVE Journal
Biologia

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JoVE Journal Biologia

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

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慢病毒介导的击倒在体外红细胞

DOI:

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14:22 min

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July 16, 2011

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Carmen G. Palii, Roya Pasha, Marjorie Brand²

¹The Sprott Center for Stem Cell Research, Regenerative Medicine Program,Ottawa Hospital Research Institute, ²Department of Cellular and Molecular Medicine,University of Ottawa

Capítulos

00:05Título
02:17Ex vivo Erythropoiesis of Human Hematopoietic Stem/Progenitor Cells
06:40Lentiviral Mediated Knockdown During ex vivo Erythroid Differentiation
10:19Results of ex vivo Erythropoiesis of Human Hematopoietic Stem Cells
13:37Conclusion

Summary

Tadução automática

一个<em>体外</em协议生成造血干/祖细胞的成熟的人体红细胞描述。此外，我们描述了一个高效的慢病毒传递方法击倒在主要的红系细胞的转录因子TAL1。使用绿色荧光蛋白表达病毒的慢病毒介导的基因传递的效率证明。

Tags

Lentiviral-mediated Knockdown Ex Vivo Erythropoiesis Human Hematopoietic Stem Cells Cell Differentiation Gene Expression Transcription Factors Lineage-specific Genes Cell Lines Erythroid Cells Stages Of Differentiation Primary HSCs Erythroid Differentiation Cord Blood Bone Marrow Adult Peripheral Blood

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