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低成本的低温光学显微镜舞台制作相关的光/电子显微镜

JoVE Journal
Bioengenharia
Author Produced

This content is Free Access.

JoVE Journal Bioengenharia

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

Please note that all translations are automatically generated. Click here for the English version.

低成本的低温光学显微镜舞台制作相关的光/电子显微镜

DOI:

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10:00 min

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June 05, 2011

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David B. Carlson, James E. Evans

¹Department of Molecular and Cellular Biology,University of California Davis

Capítulos

00:02Título
00:05Introduction
00:37Materials
01:25Fabrication
05:13Sample Preparation
06:18Using the Cryo-Light Stage
07:51Stage Stability
08:35Resultados
09:13Conclusion

Summary

Tadução automática

我们展示了一种低成本的低温阶段，设计，以适应最反射光显微镜的制造。这个实验室建成的低温阶段，实现高效，可靠的冷冻光线和低温电子显微镜之间的相关性成像。

Tags

Cryo-light Microscopy Cryo-electron Microscopy Correlated Microscopy Cellular Dynamics Ultrastructure Cryogenic Stage Cryo-LM Cryo-EM Sample Preservation Vitrification Process Correlative Imaging Inexpensive Cryogenic Stage Reflected Light Microscopes Long Working Distance Air Objectives Carbon Coated Cryo-EM Grids Multi-technique Imaging

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