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Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
 
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Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Article DOI: 10.3791/2938-v • 10:18 min • August 20th, 2011
August 20th, 2011 •
1, 1, 2, 1
1Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, 2Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology, Texas A&M University

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We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.

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Agrobacterium-mediated Virus-Induced Gene Silencing Assay Cotton Gossypium Hirsutum Molecular Breeding Gene Functional Analysis Genome Size Gene Duplication Polyploidy Growth Cycle Genetic Transformation High Throughput Functional Genetic/genomic Study VIGS Post-Transcriptional Gene Silencing (PTGS) Viral Proliferation Agrobacterium-mediated VIGS Dicots Species Solanaceae Arabidopsis Legume Species Monocots Species Barley Wheat Maize Functional Genomic Studies Tobacco Rattle Virus (TRV)
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