É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:31Insertion of Tracheal Tube
- 02:51Immune Cell Isolation
- 05:54Representative Histological, Functional and Flow-cytometric Results of Cardiac Immune Isolated Cells
- 07:05Conclusion
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
Tags
Functional Cardiac Immune CellsPathological RemodelingCardiac DiseasesMast CellsT-cellsMacrophagesBiologically Active MediatorsCytokinesProteasesTryptaseExtracellular Matrix MetabolismMatrix MetalloproteinasesCollagen AccumulationCardiac Fibroblasts’ FunctionIsolating Cardiac Immune CellsLoss Of FunctionTechnique DevelopmentUnderstanding Of Role In Cardiac DiseaseAnatomical LocationRat’s Xiphoid ProcessAxillaFalciform LigamentPericardium Of The HeartCharacterization Of FunctionalityPhenotypeMaturity
