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Journal / Immunology and Infection / Live Cell Imaging of Bacillus subtilis and…
JoVE Journal
Immunology and Infection

This Conteúdo is Open Access.

JoVE Journal Immunology and Infection
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
 

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Article DOI: 10.3791/3145-v • 07:32 min • July 28th, 2011
July 28th, 2011 •
1, 1, 1, 1
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen

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This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy. Furthermore, we provide guidelines how to analyze the microscopy images.

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Live Cell Imaging Bacillus Subtilis Streptococcus Pneumoniae Automated Time-lapse Microscopy Single Cell Studies Microscopy Method Fluorescence Microscopy Growth And Division Phylogenetic Lineage Trees Cell History And Ancestry Gene Expression Dynamics Protein Localization
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