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Probenvorbereitung, Imaging-und Analyse-Protokolle für Knife-edge-Scanning-Mikroskopie
This content is Free Access.
1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3Department of Electrical and Computer Engineering,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University
Capítulos
- 00:05Título
- 01:27Specimen Preparation: Golgi-Cox Method
- 03:20Specimen Preparation: Nissl Method
- 04:39Specimen Preparation: India Ink Method
- 05:37KESM Setup and Imaging
- 06:38Image Processing, Data Visualization and Analysis
- 08:12Representative Results of KESM
- 10:00Conclusion
Der gesamte Prozess vom Gehirn Probenvorbereitung auf serielle Schnitte Bildgebung mit dem Knife-Edge-Scanning-Mikroskop, um Daten-Visualisierung und Analyse beschrieben. Diese Technik wird derzeit verwendet, um Maus-Hirn-Daten zu erfassen, aber es ist auf andere Organe, andere Arten.
Tags
Specimen PreparationImagingAnalysis ProtocolsKnife-edge Scanning MicroscopyHigh-throughput MicroscopyHigh-resolution Microscopy3D MicroscopyNeuroanatomical DataSubmicrometer ResolutionWhole-brain-scale DataNeuronal NetworksVascular NetworksCell Body DistributionConnectomics ResearchMicrocirculation ResearchHemodynamic ResearchStereology ResearchFixingStainingEmbeddingKESM Configuration And SetupSectioning And Imaging With KESMImage ProcessingData PreparationData VisualizationData Analysis
