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Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
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Biologia
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JoVE Journal Biologia
Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

DOI:
10.3791/326-v

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11:52 min

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October 01, 2007

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,
1Department of Physiology and Biophysics,University of California, Irvine (UCI)

Capítulos

  • 00:01Título
  • 00:09Introduction
  • 02:08Preparing Reagents
  • 03:00Incubating Human Blood with RosetteSep NK Enrichment Cocktail
  • 05:15Isolating NK Cells after Histopaque Density Gradient Centrifugation
  • 11:07Conclusion

Summary

Tadução automática

Tadução automática

Natural killer cells are a small population of lymphocytes. Here we show how to isolate these cells from human blood by negative selection, using a kit from StemCell Technologies. The cells obtained are viable and untouched by antibodies, and therefore ready to be used for a number of procedures.

Tags

NK CellsRosetteSep KitStemCell TechnologiesPositive SelectionNegative SelectionSurface MarkersPeripheral BloodCytotoxic LymphocytesInnate Immune SystemCancerInfectionsPregnancyTransplant RejectionAntibody CocktailUnwanted Cell ActivationDifferentiationVideo-protocol

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