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Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
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Biologia
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JoVE Journal Biologia
Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

DOI:
10.3791/3266-v

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06:53 min

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November 23, 2011

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1Department of Neuroscience,Karolinska Institutet, 2National Institute on Drug Abuse (NIDA)

Capítulos

  • 00:05Título
  • 01:15Tissue Preparation for Cryosectioning
  • 02:01COX Histochemistry
  • 03:06SDH Histochemistry
  • 04:19Representative Examples of COX/SDH Double and Single-labeling
  • 06:22Conclusion

Summary

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The cytochrome c oxidase/sodium dehydrogenase (COX/SDH) double-labeling method allows for direct visualization of mitochondrial respiratory enzyme deficiencies in fresh-frozen tissue sections. This is a straightforward histochemical technique and is useful in investigating mitochondrial diseases, aging, and aging-related disorders.

Tags

Mitochondrial Respiratory FunctionCytochrome C OxidaseSuccinate DehydrogenaseCOX/SDH Double-labeling HistochemistryMitochondrial DNA DefectsMtDNA MutationsBioenergetics HomeostasisCellular FunctionMitochondrial Theory Of AgingMtDNA DamageRespiratory EnzymesCytochrome C Oxidase (COX)MtDNA IntegritySuccinate Dehydrogenase (SDH)Nuclear DNAMitochondrial DiseasesAgingAge-related Diseases

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