Journal
/
Biologia
/
A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

DOI:
10.3791/3394-v

•

05:19 min

•

January 16, 2012

•

,
1Biosciences, College of Life and Environmental Sciences,The University of Exeter

Capítulos

  • 00:05Título
  • 01:54Mounting Leaf Samples in PFD
  • 03:20Representative Leaf Imaging Results
  • 04:37Conclusion

Summary

Tadução automática

Tadução automática

We describe the use of perfluorodecalin as an infiltrative mounting medium. This is a simple method for improving depth of imaging in Arabidopsis thaliana leaf tissue with minimal physiological impact.

Tags

Arabidopsis LeavesImagingPerfluorodecalinInfiltrative Imaging MediumHigh-resolution ImagesRefractive IndexBiological SamplesSpongy MesophyllPlant LeavesVertebrate LungsFluorescence MicroscopyPerfluorocarbonLaser Scanning Confocal MicroscopyPFD ProtocolOptical ComplexityWater Refractive IndexCytosol Refractive IndexNon-fluorescentNon-toxicSurface Tension

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/3394?article_type=v

Pyrosequencing: A Simple Method for Accurate Genotyping
check_url/pt/3394?article_type=v

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
check_url/pt/3394?article_type=v

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
check_url/pt/3394?article_type=v

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
check_url/pt/3394?article_type=v

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
check_url/pt/3394?article_type=v

FRET Imaging in Three-dimensional Hydrogels
check_url/pt/3394?article_type=v

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
check_url/pt/3394?article_type=v

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
check_url/pt/3394?article_type=v

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
check_url/pt/3394?article_type=v

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Read Article