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Imunologia e Infecção
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Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics

Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics

DOI:
10.3791/3438-v

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08:08 min

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December 08, 2011

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, ,
1Department of Physiology, School of Medicine,Louisiana State University Health Sciences Center

Capítulos

  • 00:05Título
  • 01:51Collecting Lymphatic Isolation
  • 03:36Ratiometric Measurement of [Ca2+]
  • 04:46Pressure Control Protocol
  • 05:51[Ca2+] in Isolated Lymphatics
  • 07:42Conclusion

Summary

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We introduce an approach to evaluate the cytosolic Ca2+ concentration in isolated lymphatics to study Ca2+-dependent and Ca2+-sensitizing mechanisms of lymphatic smooth muscle contraction.

Tags

Cytosolic Ca2+Isolated Contractile LymphaticsLymphatic VesselsFluid HomeostasisLipidsCentral CirculationSurveillance SystemAntigensMucosal ImmunityAdaptive Immune ResponsesInterstitial FluidInitial LymphaticsPressure GradientCollecting LymphaticsLymphangionsBicuspid ValvesBackflow PreventionContractile CyclePhasic Contractile PatternCardiac CycleSystolic PhaseDiastolic PhaseContraction FrequencyLymphatic Smooth MuscleTone GenerationMyogenic ConstrictionMyogenic DilationLuminal Pressure Increases And DecreasesMolecular MechanismsCytosolic Ca2+ Concentration

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