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JoVE Journal Bioengineering
Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
 
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Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Article DOI: 10.3791/3441-v • 14:19 min • October 4th, 2011
October 4th, 2011 •
1, 2, 2, 2, 1
1Department of Chemistry, Gottwald Center for the Sciences, University of Richmond, 2Department of Biochemistry and Molecular Biology, Gottwald Center for the Sciences, University of Richmond

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Alkanethiolate stabilized gold colloids known as monolayer protected clusters (MPCs) are synthesized, characterized, and assembled into thin films as an adsorption interface for protein monolayer electrochemistry of simple redox protein like Pseudomonas aeruginosa azurin (AZ) and cytochrome c (cyt c).

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Monolayer Protected Gold Nanoparticles Alkanethiolate Ligands Film Assemblies Protein Monolayer Electrochemistry Adsorption Platforms Redox Proteins Electron Transfer Reactions Gold Nanoparticle Film Assemblies Self-assembled Monolayers Brust Reaction UV-Vis Spectroscopy Transmission Electron Microscopy Proton NMR Dip Cycle Method Dithiol Linking Molecules Gold Electrode Interfaces Film Growth
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