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JoVE Journal Neuroscience
Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration
 

Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration

Article DOI: 10.3791/3516-v • 08:47 min • February 10th, 2012
February 10th, 2012 •
1, 1
1Department of Biology, University of Victoria

Capítulos

Resumo
Automatic Translation

We have developed a novel technique of quantifying nicotinic acetylcholine receptor changes within subcellular regions of specific subtypes of CNS neurons to better understand the mechanisms of nicotine addiction by using a combination of approaches including fluorescent protein tagging of the receptor using the knock-in approach and spectral confocal imaging.

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Spectral Confocal Imaging Fluorescently Tagged Nicotinic Receptors Knock-in Mice Chronic Nicotine Administration Ligand-gated Ion Channels Central Nervous System (CNS) Addiction To Nicotine Ion Channel Distribution Chronic Nicotine Exposure Nicotinic Acetylcholine Receptors (nAChRs) Brain Tissue Glutamatergic GluN1 GluA1 Channels Sensitization To Addictive Drugs Map And Quantify Distribution Expression Patterns Specific Ion Channels Mechanisms Of Addiction Brain Region-specific Effects Radioactive Ligands Low Spatial Resolution Ligand-gated Ion Channels In Neurons Genetically Encoded Fluorescent Reporters
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