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生殖细胞的移植和睾丸组织在小鼠异种移植
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Biologia
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JoVE Journal Biologia
Germ Cell Transplantation and Testis Tissue Xenografting in Mice
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生殖细胞的移植和睾丸组织在小鼠异种移植

DOI:
10.3791/3545-v

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10:41 min

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February 06, 2012

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, ,
1Department of Comparative Biology and Experimental Medicine,University of Calgary

Capítulos

  • 00:05Título
  • 03:11Germ Cell-transplantation Procedure
  • 05:32Preparation of Donor Tissue for Xenotransplantation
  • 06:57Castration of Recipient Mouse and Ectopic Xenografting
  • 08:48Transplanted SSCs and Xenograft Development
  • 09:54Conclusion

Summary

Tadução automática

Tadução automática

生殖细胞移植和睾丸组织异种移植的协议描述。在捐助源性受援国睾丸精子的生殖细胞移植的结果和鉴定精原干细胞（SSCS）为代表的功能重建法。睾丸组织异种移植在受体小鼠睾丸发育和精子各捐助国物种再现。

Tags

Germ Cell TransplantationMiceDr. Ralph BrinsterUniversity Of PennsylvaniaMicroinjectionSeminiferous TubulesSpermatogenesisSperm ProductionDonor-derivedBacterial β-galactosidase GeneOffspringLumen Of The Seminiferous TubulesBasement MembraneSpermatogoniaSSCsStem Cell NichePutative Spermatogonial Stem CellsTransgenic AnimalsGenetic ManipulationSertoli Cell-germ Cell InteractionSSC Homing And ColonizationSelf-renewal And Differentiation

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