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MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model
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JoVE Journal Medicina
MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

DOI:
10.3791/3555-v

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07:42 min

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March 13, 2012

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, , ,
1Imaging Research,Sunnybrook Research Institute, 2Department of Medical Biophysics,University of Toronto, 3Department of Medical Biophysics, and Institute of Biomaterials & Biomedical Engineering (IBBME),University of Toronto

Capítulos

  • 00:05Título
  • 01:37Ultrasound and MRI Setup
  • 02:08Animal Preparation
  • 03:19Target Selection
  • 03:45Microbubble Preparation
  • 04:41Ultrasound Delivery
  • 05:11MRI Evaluation of Treatment Outcome
  • 05:45Delivery of MRI Contrast Agents Using Focused Ultrasound (FUS) and Circulating Microbubbles
  • 07:17Conclusion

Summary

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Microbubble-mediated focused ultrasound disruption of the blood-brain barrier (BBB) is a promising technique for non-invasive targeted drug delivery in the brain1-3. This protocol outlines the experimental procedure for MRI-guided transcranial BBB disruption in a rat model.

Tags

MRI-guidedDisruptionBlood-brain BarrierTranscranial Focused UltrasoundRat ModelFUSPharmaceutical TreatmentsBrain DisordersMoleculesVasculatureSizeTemporaryReversibleChemical MeansLocalizedTherapeutic AgentsConcentrationUltrasound ParametersExperimental ProcedureAccurate TargetingAnimal PreparationMicrobubble Handling

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