Journal
/
Biologia
/
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

DOI:
10.3791/3562-v

•

07:56 min

•

January 17, 2012

•

, ,
1Department of Orthopaedic Surgery,NYU Hospital for Joint Diseases, 2Department of Cell Biology,New York University School of Medicine

Capítulos

  • 00:05Título
  • 01:07Small Scale Transformation of Bait Plasmid
  • 02:12Bait Validation
  • 03:09cDNA Library Screening
  • 05:06X-gal Assay
  • 05:59Retransformation in the MY2H Assay
  • 06:47Isolation of Progranulin-Interacting Proteins
  • 07:32Conclusion

Summary

Tadução automática

Tadução automática

We have modified the conventional yeast two-hybrid screening, an effective genetic tool in identifying protein interaction. This modification markedly shortens the process, reduces the workload, and most importantly, reduces the number of false positives. In addition, this approach is reproducible and reliable.

Tags

Modified Yeast-two-hybrid SystemProteinsInteractingGrowth FactorProgranulinPGRNGranulin Epithelin PrecursorAutocrine Growth FactorPhysiologic ProcessesDisease ProcessesInflammationHost DefenseNeurotrophic FactorPGRN GeneFrontotemporal DementiaCartilage Development And DegradationBinding Receptor(s)Modified Yeast Two-hybrid ApproachGAL4 Based 2-hybrid SystemFalse Positive ClonesReproducible And Reliable System

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/3562?article_type=v

Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants
check_url/pt/3562?article_type=v

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos
check_url/pt/3562?article_type=v

Preparing T Cell Growth Factor from Rat Splenocytes
check_url/pt/3562?article_type=v

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
check_url/pt/3562?article_type=v

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
check_url/pt/3562?article_type=v

PAR-CliP – A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
check_url/pt/3562?article_type=v

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
check_url/pt/3562?article_type=v

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
check_url/pt/3562?article_type=v

Capture Compound Mass Spectrometry – A Powerful Tool to Identify Novel c-di-GMP Effector Proteins
check_url/pt/3562?article_type=v

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Read Article