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Capítulos
- 00:05Título
- 01:07Small Scale Transformation of Bait Plasmid
- 02:12Bait Validation
- 03:09cDNA Library Screening
- 05:06X-gal Assay
- 05:59Retransformation in the MY2H Assay
- 06:47Isolation of Progranulin-Interacting Proteins
- 07:32Conclusion
Nous avons modifié la levure classique double-hybride en dépistage, un outil efficace dans l'identification génétique d'interactions protéiques. Cette modification raccourcit nettement le processus, réduit la charge de travail, et surtout, réduit le nombre de faux positifs. En outre, cette approche est reproductible et fiable.
Tags
Modified Yeast-two-hybrid SystemProteinsInteractingGrowth FactorProgranulinPGRNGranulin Epithelin PrecursorAutocrine Growth FactorPhysiologic ProcessesDisease ProcessesInflammationHost DefenseNeurotrophic FactorPGRN GeneFrontotemporal DementiaCartilage Development And DegradationBinding Receptor(s)Modified Yeast Two-hybrid ApproachGAL4 Based 2-hybrid SystemFalse Positive ClonesReproducible And Reliable System
