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Imunologia e Infecção
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Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

DOI:
10.3791/3609-v

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11:36 min

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January 14, 2012

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, , ,
1Department of poverty related diseases,Barcelona Centre for International Health Research, 2Confocal Microscopy Unit,University of Barcelona- Scientific and Technological Centers, 3Institució Catalana de Recerca i Estudis Avançats (ICREA)

Capítulos

  • 00:05Título
  • 01:52Animal Infection
  • 03:33Ex Vivo Labeling of RBCs
  • 04:30Surgical Procedures
  • 09:184D Imaging of Parasite Mobility
  • 10:12Blood Flow
  • 10:55Conclusion

Summary

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We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.

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Intravital MicroscopySpleenQuantitative AnalysisParasite MobilityBlood FlowMalaria ModelsParasite-host InteractionsPre-erythrocytic StagesSkin Lymph NodesHepatocyte-derived MerosomeMerozoitesErythrocytesProtein ExportPlasmodium DevelopmentDynamic PassageLymphoid OrganInfected Red Blood CellsPlasmodium YoeliiBalb/c MiceBarrier CellsFibroblastic OriginRed Pulp

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