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Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles
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Bioengenharia
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JoVE Journal Bioengenharia
Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles

Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles

DOI:
10.3791/3883-v

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11:26 min

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June 08, 2012

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, , , ,
1Department of Chemical and Biological Engineering,Iowa State University, 2Department of Veterinary Microbiology and Preventive Medicine,Iowa State University

Capítulos

  • 00:05Título
  • 01:40Harvesting Alveolar Macrophages from the Mouse using Lung Lavage
  • 04:26Processing of Lung Lavage Harvest
  • 06:29Addition of Polyanhydride Nanoparticles and Control Treatments
  • 08:59Results from Flow-cytometric Analysis of Nanoparticle-induced Activation of Isolated Alveolar Macrophages
  • 10:46Conclusion

Summary

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Herein, we describe protocols for harvesting murine alveolar macrophages, which are resident innate immune cells in the lung, and examining their activation in response to co-culture with polyanhydride nanoparticles.

Tags

Murine Alveolar MacrophagesCellular ActivationPolyanhydride NanoparticlesIntranasal VaccinesRespiratory Infectious DiseasesSebacic Acid16-bis(p-carboxyphenoxy)hexane18-bis(p-carboxyphenoxy)-36-dioxaoctaneBulk Erosion KineticsSurface Erosion KineticsBiomolecules ReleaseAdjuvant ActivityVaccine Delivery PlatformInnate ImmunityMolecular ResponsesCellular ResponsesAntigen Presenting Cells (APCs)Dendritic CellsAlveolar Macrophages (AM)Lung ParenchymaMicrobial PathogensCell Debris

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