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Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
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Neurociência
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JoVE Journal Neurociência
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

DOI:
10.3791/3907-v

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09:51 min

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July 15, 2012

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, ,
1Fishberg Department of Neuroscience and Friedman Brain Institute,Mt. Sinai School of Medicine, 2Phase Five Communications Inc.

Capítulos

  • 00:05Título
  • 01:53Dissection
  • 03:21Preparing electrodes
  • 04:40Loading the calcium indicator dye
  • 05:27Calcium imaging and electrophysiological recording
  • 07:57Analysis
  • 08:40Representative data
  • 09:18Conclusions

Summary

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We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.

Tags

Intracellular CalciumSynaptic EfficacyMollusc AplysiaCalcium ImagingIntracellular RecordingBuccal GanglionSynaptic PlasticityHomosynaptic FacilitationCalcium OrangeIontophoresisFluorescent Dye

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