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监测变化的细胞内钙离子浓度和突触效能的软体动物<em>海兔</em

JoVE Journal
Neurociência
Author Produced

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JoVE Journal Neurociência

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

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监测变化的细胞内钙离子浓度和突触效能的软体动物<em>海兔</em

DOI:

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09:51 min

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July 15, 2012

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Bjoern Ch. Ludwar, Colin G. Evans², Elizabeth C. Cropper

¹Fishberg Department of Neuroscience and Friedman Brain Institute,Mt. Sinai School of Medicine, ²Phase Five Communications Inc.

Capítulos

00:05Título
01:53Dissection
03:21Preparing electrodes
04:40Loading the calcium indicator dye
05:27Calcium imaging and electrophysiological recording
07:57Analysis
08:40Representative data
09:18Conclusions

Summary

Tadução automática

我们演示了如何在细胞内游离钙离子浓度和突触效能的变化，可以同时监测神经节准备的<em>海兔</em>。我们的图像使用的荧光染料，钙奥兰治，用锋利的（细胞内）电极和诱导和监测突触传递的细胞内钙离子。

Tags

Intracellular Calcium Synaptic Efficacy Mollusc Aplysia Calcium Imaging Intracellular Recording Buccal Ganglion Synaptic Plasticity Homosynaptic Facilitation Calcium Orange Iontophoresis Fluorescent Dye

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