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一个循序渐进的步骤方法的到Nanodisc脂质颗粒的ABC转运蛋白的重组
JoVE Journal
Biologia
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JoVE Journal Biologia
A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
DOI:

12:25 min

August 31, 2012

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Capítulos

  • 00:05Título
  • 02:08Preparation of the Membrane Scaffold Protein, MSP
  • 03:53Preparation of the MalFGK2 Complex
  • 05:09Preparation of Phospholipids
  • 06:07Preparation of Bio-Beads & Nanodisc Reconstitution
  • 07:59Nanodisc Analysis: Native Gel Electrophoresis
  • 08:26Nanodisc Analysis: Dynamic Light Scattering (DLS)
  • 09:11Nanodisc Analysis: ATPase Measurements
  • 09:47Results: Activity of the maltose Transporter Reconstituted into Nanodiscs
  • 11:15Conclusion

Summary

Tadução automática

奈米圆盘是小盘状颗粒结合成一小片的磷脂双分子层膜蛋白。我们提供了一个可视化的协议,一步一步注册成立的MalFGK2转运到光盘。

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