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Isolation and Primary Culture of Rat Hepatic Cells
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Biologia
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JoVE Journal Biologia
Isolation and Primary Culture of Rat Hepatic Cells

Isolation and Primary Culture of Rat Hepatic Cells

DOI:
10.3791/3917-v

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07:06 min

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June 29, 2012

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, , ,
1Department of Pathology and Laboratory Medicine,University of Cincinnati College of Medicine, 2American University in Washington, D.C., 3Department of Internal Medicine,Saint Louis University School of Medicine

Capítulos

  • 00:05Título
  • 01:04Rat Perfusion for Liver Isolation
  • 03:26Hepatocyte Cell Isolation
  • 04:42Hepatocyte Culture
  • 06:09Typical Hepatocyte Harvest and 24-hour Culture Results
  • 06:44Conclusion

Summary

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Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.

Tags

IsolationPrimary CultureRat HepatocytesCollagenase PerfusionLiver FunctionDiseasePathophysiologyPharmacologyPesquisaTechniqueSeglenDissociationPortal VeinNylon FilterCulture PlatesSerum-containing MediumSerum-free MediumSurgical StepsSterile Culture StepsVideo Demonstration

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